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Related Experiment Video

Updated: Jun 26, 2026

Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
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SCHISTOSOME OVA: RAPID SEPARATION FROM MOUSE TISSUE BY DIGESTION WITH PINGUINAIN.

E TORO-GOYCO, E RIVERA-COLLAZO, R RODRIGUEZ-MOLINA

    Science (New York, N.Y.)
    |October 18, 1963
    PubMed
    Summary

    Pinguinain enzyme effectively digests Schistosoma mansoni infected mouse tissues, yielding high numbers of parasite ova. This method aids in Schistosomiasis research by improving parasite recovery from host tissues.

    Keywords:
    EXPERIMENTAL LAB STUDYINTESTINAL DISEASES, PARASITICLIVER DISEASES, PARASITICMICEPEPTIDE HYDROLASESSCHISTOSOMIASIS

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    Area of Science:

    • Parasitology
    • Biochemistry
    • Infectious Diseases

    Background:

    • Schistosomiasis mansoni is a significant parasitic disease.
    • Accurate quantification of Schistosoma mansoni ova is crucial for research and diagnosis.
    • Current methods for ova recovery can be labor-intensive and yield variable results.

    Purpose of the Study:

    • To evaluate the efficacy of the proteolytic enzyme pinguinain for extracting Schistosoma mansoni ova.
    • To establish a reliable method for high-yield ova recovery from infected host tissues.

    Main Methods:

    • Homogenization of liver and intestine tissues from Schistosoma mansoni infected mice.
    • Digestion of homogenized tissues using the proteolytic enzyme pinguinain.
    • Quantification of recovered Schistosoma mansoni ova.

    Main Results:

    • High yields of Schistosoma mansoni ova were successfully obtained.
    • Pinguinain digestion proved effective in releasing ova from host tissues.
    • The method demonstrated efficiency in parasite recovery.

    Conclusions:

    • Pinguinain is a potent enzyme for the digestion of host tissues in Schistosomiasis research.
    • This enzymatic digestion method offers a high-yield approach for Schistosoma mansoni ova recovery.
    • The findings support the use of pinguinain for improved parasite quantification in experimental settings.