Related Experiment Video
Updated: Sep 9, 2026
Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun
Published on: April 18, 2014
Cloning, nucleotide sequence determination and expression of the genes encoding cytochrome P-450soy (soyC) and
M K Trower1, R Lenstra, C Omer
1Central Research and Development Department, E. I. du Pont de Nemours and Company, Inc., Experimental Station, Wilmington, Delaware 19880-0228.
Abstract:
Xenobiotic transformation by Streptomyces griseus (ATCC13273) is catalysed by a cytochrome P-450, designated cytochrome P-450soy. A DNA segment carrying the structural gene encoding P-450soy (soyC) was cloned using an oligonucleotide probe constructed from the protein sequence of a tryptic peptide. Following DNA sequencing the deduced amino acid sequence of P-450soy was compared with that for P-450cam, revealing conservation of important structural components including the haem pocket. Expression of the cloned soyC gene product was demonstrated in Streptomyces lividans by reduced CO:difference spectral analysis and Western blotting. Downstream of soyC, a gene encoding a putative [3Fe-4S] ferredoxin (soyB), named ferredoxinsoy, was identified.
