Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Stress-induced proteolysis in yeast.

W Hilt1, D H Wolf

  • 1Institut für Biochemie, Universität Stuttgart, Germany.

Molecular Microbiology
|September 1, 1992
PubMed
Summary

Cellular survival relies on adapting to environmental changes. Yeast utilize two key proteolytic systems—vacuolar and non-vacuolar—to eliminate harmful or damaged proteins during stress, ensuring survival.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Common and dissociable regional cerebral blood flow differences associate with dimensions of psychopathology across categorical diagnoses.

Molecular psychiatry·2017
Same author

Prefrontal cortical thinning links to negative symptoms in schizophrenia via the ENIGMA consortium.

Psychological medicine·2017
Same author

Cortical abnormalities in bipolar disorder: an MRI analysis of 6503 individuals from the ENIGMA Bipolar Disorder Working Group.

Molecular psychiatry·2017
Same author

Positive symptoms associate with cortical thinning in the superior temporal gyrus via the ENIGMA Schizophrenia consortium.

Acta psychiatrica Scandinavica·2017
Same author

Subcortical brain volume abnormalities in 2028 individuals with schizophrenia and 2540 healthy controls via the ENIGMA consortium.

Molecular psychiatry·2015
Same author

Connectome-wide network analysis of youth with Psychosis-Spectrum symptoms.

Molecular psychiatry·2015

Area of Science:

  • Cellular biology
  • Molecular biology
  • Biochemistry

Background:

  • Cellular survival depends on adapting to environmental changes.
  • Proteolysis is a critical cellular mechanism for stress response.
  • Eliminating damaged or harmful proteins is essential under stress.

Purpose of the Study:

  • To investigate the role of proteolytic systems in yeast stress response.
  • To elucidate the mechanisms of protein degradation during cellular stress.
  • To highlight the importance of both vacuolar and non-vacuolar proteolysis.

Main Methods:

  • Studied the yeast Saccharomyces cerevisiae as a model eukaryote.
  • Characterized the vacuolar (lysosomal) proteolytic system.
  • Investigated the non-vacuolar proteolytic system, including proteinase yscE.

Main Results:

  • Yeast possess two distinct proteolytic systems: vacuolar and non-vacuolar.
  • The vacuolar system involves non-specific peptidases within a membrane-enveloped compartment.
  • The non-vacuolar system includes highly specific peptidases, such as proteinase yscE (proteasome equivalent).

Conclusions:

  • Both the vacuolar and non-vacuolar proteolytic systems are vital for yeast survival under stress.
  • These systems work in concert to manage protein homeostasis during adverse conditions.
  • Understanding these pathways is crucial for comprehending eukaryotic stress adaptation.

Related Experiment Videos