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Updated: Aug 10, 2026
14:44
Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
Published on: December 16, 2013
Manganese + 2 exhibits dynamic binding to multiple ligands in human plasma
1Department of Nutrition, University of California, Davis 95616.
Metabolism: Clinical and Experimental
|October 1, 1992
Summary
This study investigated manganese (Mn) in human plasma, finding it primarily binds to very low molecular weight proteins. Manganese also associates with transferrin (Tf) over time, suggesting oxidation is key to its plasma transport.
Area of Science:
- Biochemistry
- Trace Element Metabolism
- Human Physiology
Background:
- Manganese is an essential trace element crucial for various biological functions.
- Understanding manganese transport and localization in human plasma is vital for its metabolic studies.
- Previous research has not fully elucidated the specific binding proteins and forms of manganese in plasma.
Purpose of the Study:
- To investigate the molecular localization and binding characteristics of manganese in human plasma.
- To explore the role of oxidation state in manganese-transferrin interaction.
- To identify the primary carriers of manganese in the plasma of fasted adult males.
Main Methods:
- In vitro labeling of plasma with radioactive 54MnCl2.
- Fractionation using molecular sieve chromatography and affinity chromatography with anti-transferrin antibodies.
- Analysis of manganese distribution among different molecular weight fractions.
- Electrophoretic analysis of plasma components.
Main Results:
- The majority of 54Mn was found in a very low molecular weight (VLMW) fraction.
- Significant 54Mn peaks were also observed associated with transferrin (Tf) and other uncharacterized fractions.
- The association of 54Mn with Tf increased over incubation time, indicating a time-dependent binding process.
- Evidence suggests Mn+2 oxidation to Mn+3 precedes or facilitates binding to Tf.
- Electrophoresis provided inconclusive results for plasma manganese localization.
Conclusions:
- Plasma manganese is predominantly associated with VLMW species, not solely with transferrin.
- Manganese binding to transferrin is a time-dependent process influenced by manganese oxidation state.
- Further research is needed to fully characterize the VLMW manganese-binding species and the overall manganese metabolic pathway in human plasma.
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