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[Immunologic and electrophoretic detection of an epidermal chalone-containing complex in tissues of different origin]
The rabbit antiserum to one of the components of the tissue-specific protein complex isolated from the back skin of rats and containing G1- and G2-chalones was obtained. By means of this antiserum an antigen identical to the cutaneous one was found in the mucous membrane of the tongue, oesophagus, prestomach and vagina, in the epidermis of the tail and sole. The cornea, mucous membranes of the urinary bladder and intestine, liver, kidney and blood serum do not contain this antigen. According to data of disc-electrophoresis in 5%-polyacrilamide gel, the 55--81% alcohol extracts of the investigated tissues of the epidermal type, including the cornea and mucous membrane of the urinary bladder, are of similar quantitative and qualitative composition, different from that of tissues of other origin.
The rabbit antiserum to one of the components of the tissue-specific protein complex isolated from the back skin of rats and containing G1- and G2-chalones was obtained. By means of this antiserum an antigen identical to the cutaneous one was found in the mucous membrane of the tongue, oesophagus, prestomach and vagina, in the epidermis of the tail and sole. The cornea, mucous membranes of the urinary bladder and intestine, liver, kidney and blood serum do not contain this antigen. According to data of disc-electrophoresis in 5%-polyacrilamide gel, the 55--81% alcohol extracts of the investigated tissues of the epidermal type, including the cornea and mucous membrane of the urinary bladder, are of similar quantitative and qualitative composition, different from that of tissues of other origin.