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Related Experiment Videos

The ARS consensus sequence is required for chromosomal origin function in Saccharomyces cerevisiae.

A M Deshpande1, C S Newlon

  • 1Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey-New Jersey Medical School, Newark 07103-2174.

Molecular and Cellular Biology
|October 1, 1992
PubMed
Summary
This summary is machine-generated.

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Chromosomal replication origins rely on ARS elements. Deleting or mutating ARS elements on chromosomes confirmed they are essential for DNA replication origin function, not just plasmid stability.

Area of Science:

  • Molecular Biology
  • Genetics
  • Yeast Biology

Background:

  • Replication origins are crucial for DNA replication, and in yeast, these sites are known as autonomously replicating sequences (ARS).
  • Previous studies mapped replication origins to ARS elements, but their direct necessity for chromosomal origin function remained unclear.

Purpose of the Study:

  • To investigate whether the DNA sequences essential for ARS function in plasmids are also required for the function of chromosomal replication origins.
  • To determine if ARS elements themselves confer chromosomal origin function.

Main Methods:

  • Deletion of the chromosomal ARS306 element.
  • Replacement of the chromosomal ARS307 element with mutant derivatives containing point mutations in domain A of the ARS core consensus sequence.

Related Experiment Videos

  • Assay of chromosomal origin function using two-dimensional agarose gel electrophoresis.

    • Main Results:

    • Deletion of ARS306 resulted in the loss of the associated chromosomal replication origin.
    • Mutations in domain A of ARS307 affected chromosomal origin function in a manner similar to their effects on ARS function measured by plasmid stability.
    • These findings indicate a direct correlation between ARS element integrity and chromosomal origin activity.

    Conclusions:

    • Chromosomal replication origin function is an intrinsic property of the ARS element itself.
    • The DNA sequences within ARS elements are necessary for initiating DNA replication at chromosomal origins.