Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Laminin binding to Prevotella intermedia.

S Kalfas1, Z Tigyi, M Wikström

  • 1University of Göteborg, Sweden.

Oral Microbiology and Immunology
|August 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gingival thickness and gingival width in children: a cross-sectional study utilizing ultrasonography.

European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry·2024
Same author

Low health-related quality of life in adult individuals with multiple limb deficiencies compared with population-based reference values.

Prosthetics and orthotics international·2022
Same author

Low prevalence of multi-resistant bacteria in undergraduate dental students; an observational case-control multi-centre study in Europe.

Journal of oral microbiology·2021
Same author

Infection of Potatoes by Oospores of Phytophthora infestans in Soil.

Plant disease·2019
Same author

First Report in Sweden of Downy Mildew on Parsley Caused by Plasmopara petroselini.

Plant disease·2019
Same author

Root Rot of Pea and Faba Bean in Southern Sweden Caused by Phytophthora pisi sp. nov.

Plant disease·2019
Same journal

Influence of mouse prolactin-inducible protein in saliva on the aggregation of oral bacteria.

Oral microbiology and immunology·2009
Same journal

Detection of herpetic viruses in gingival crevicular fluid of patients suffering from periodontal diseases: prevalence and effect of treatment.

Oral microbiology and immunology·2009
Same journal

Characterization of bacterial flora in persistent apical periodontitis lesions.

Oral microbiology and immunology·2009
Same journal

Genetic diversity and toxic activity of Aggregatibacter actinomycetemcomitans isolates.

Oral microbiology and immunology·2009
Same journal

Characterization of L-cysteine desulfhydrase from Prevotella intermedia.

Oral microbiology and immunology·2009
Same journal

Differential virulence and innate immune interactions of Type I and II fimbrial genotypes of Porphyromonas gingivalis.

Oral microbiology and immunology·2009
See all related articles

Prevotella intermedia, a bacterium found in diseased periodontal pockets, binds to laminin (Lm) via surface proteins. This interaction, influenced by pH, may aid bacterial colonization in the periodontium.

Area of Science:

  • Microbiology
  • Oral Biology
  • Bacterial Pathogenesis

Background:

  • Laminin (Lm) is a key basement membrane protein in the periodontium.
  • Prevotella intermedia is frequently isolated from diseased periodontal pockets.
  • Bacterial interactions with host matrix proteins can facilitate colonization and disease progression.

Purpose of the Study:

  • To investigate the binding interaction between Prevotella intermedia and laminin.
  • To characterize the properties of this interaction and identify the binding component.

Main Methods:

  • 125I-labeled laminin binding assay with 66 Prevotella intermedia strains.
  • Characterization of binding kinetics, pH dependence, and stability.
  • Sodium dodecylsulfate-polyacrylamide gel electrophoresis and Western blot analysis.

Related Experiment Videos

Main Results:

  • Prevotella intermedia demonstrated significant binding to laminin, with mean binding of 28%.
  • Binding increased at lower pH (pH 6) and was optimal between pH 3.0-6.5.
  • A heat-labile, protease-sensitive ~62 kDa protein on the bacterial surface was identified as the primary laminin-binding component.

Conclusions:

  • Prevotella intermedia possesses cell surface proteins that interact with laminin.
  • This laminin-binding capability may contribute to the bacterium's ability to colonize the periodontal pocket.
  • Understanding this interaction offers potential targets for therapeutic interventions.