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A Method for Growing Bio-memristors from Slime Mold
07:46

A Method for Growing Bio-memristors from Slime Mold

Published on: November 2, 2017

NUTRITION OF CELLULAR SLIME MOLDS. III. SPECIFIC GROWTH REQUIREMENTS OF POLYSPHONDYLIUM PALLIDUM

H HOHL, K B RAPER

    Journal of Bacteriology
    |December 1, 1963
    PubMed

    Insights

    This study details axenic growth media for Polysphondylium pallidum cellular slime molds. Researchers developed both complex and defined media, identifying specific nutrient requirements for optimal myxamoebae proliferation.

    Area of Science:

    • Microbiology
    • Cell Biology
    • Mycology

    Background:

    • Cellular slime molds, including Polysphondylium pallidum, are important model organisms in developmental biology.
    • Axenic culture is crucial for detailed study of their nutritional requirements and life cycle.

    Purpose of the Study:

    • To describe soluble media for the axenic growth of Polysphondylium pallidum.
    • To identify specific nutritional requirements for optimal growth.

    Main Methods:

    • Development of a complex medium using tryptose and serum albumin.
    • Supplementation of the complex medium with various organic compounds (amino acids, vitamins, nucleic acid bases, glucose).
    • Development of a defined medium with specific amino acids, riboflavin, purines, pyrimidines, dextrose, and trace elements.

    Main Results:

    • A complex medium achieved cell densities up to 10(7) myxamoebae/ml.
    • Supplementation of the complex medium increased growth to 3 x 10(7) cells/ml with a 4.5-hour generation time.
    • A defined medium supported growth of up to 5.5 x 10(6) myxamoebae/ml.

    Conclusions:

    • Specific amino acids, vitamins, nucleic acid bases, and carbohydrates are essential for Polysphondylium pallidum growth.
    • Defined media can support axenic culture, enabling detailed nutritional studies of cellular slime molds.
    Keywords:
    AMINO ACIDSCULTURE MEDIAEXPERIMENTAL LAB STUDYGLUCOSEMETABOLISMMYXOMYCETESSALTSSERUM ALBUMIN, BOVINETRACE ELEMENTSVITAMINS

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