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A perfused-cuvette method for fluorimetric studies of non-adherent cells
L C Schlichter1, P S Pennefather, C J van Staden
1Playfair Neuroscience Unit, Toronto Hospital, Ontario, Canada.
Pflugers Archiv : European Journal of Physiology
|July 1, 1992
Abstract:
We have developed a system for immobilizing non-adherent cells for use in macroscopic fluorescence measurements in a perfused cuvette. Using normal human T lymphocytes loaded with the fluorescent Ca(2+)-indicator, fluo-3, we have validated the properties of these cells immobilized on clear, non-fluorescent fluorohalocarbon film (Aclar) using the non-charged cell adhesive, Cell-Tak.