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RHEUMATOID FACTOR PROPERTIES OF HYPERIMMUNE RABBIT SERA

C L CHRISTIAN

    The Journal of Experimental Medicine
    |November 1, 1963
    PubMed

    Insights

    This study identifies a rheumatoid factor-like substance (RFLS) exclusively in the macroglobulin fraction of rabbit sera. This substance, separable by various methods, shows selective reactivity with antibodies.

    Area of Science:

    • Immunology
    • Biochemistry

    Background:

    • Hyperimmunization of rabbits with bacteria can induce specific serum factors.
    • Understanding the nature and properties of induced serum substances is crucial in immunology.

    Purpose of the Study:

    • To characterize the rheumatoid factor-like substance (RFLS) induced by bacterial hyperimmunization in rabbits.
    • To determine the serum fraction and properties associated with RFLS.

    Main Methods:

    • Zone centrifugation
    • DEAE cellulose chromatography
    • Gel filtration
    • Immunoelectrophoretic analysis
    • Analytical ultracentrifugation
    • Pepsin treatment of gamma-globulin

    Main Results:

    • RFLS was consistently and exclusively associated with the macroglobulin fraction of rabbit sera.
    • RFLS was successfully separated from bulk serum proteins using multiple biochemical techniques.
    • Partially purified RFLS exhibited selective reactivity with rabbit isohemagglutinin antibodies.
    • Pepsin treatment significantly reduced or eliminated the reactivity of rabbit gamma-globulin with RFLS.

    Conclusions:

    • The rheumatoid factor-like substance (RFLS) is a macroglobulin with specific antibody-binding properties.
    • RFLS characterization provides insights into the immune response to bacterial antigens in rabbits.
    Keywords:
    AGGLUTINATIONBACILLUS SUBTILISESCHERICHIA COLIEXPERIMENTAL LAB STUDYGAMMA GLOBULINIMMUNE SERUMSIMMUNOELECTROPHORESISPEPSINRABBITSRHEUMATOID FACTORSALMONELLA TYPHIMURIUM

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