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Related Experiment Video

Updated: Jun 18, 2026

Functional Cloning Using a Xenopus Oocyte Expression System
09:40

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Published on: January 30, 2016

CELL WALL REPLICATION IN SALMONELLA TYPHOSA.

R M COLE

    Science (New York, N.Y.)
    |February 21, 1964
    PubMed
    Summary

    Salmonella typhosa cell wall growth was studied using fluorescence. New wall material is incorporated diffusely, unlike Streptococcus pyogenes, even when cell division is inhibited by chloramphenicol.

    Area of Science:

    • Microbiology
    • Cell Biology
    • Bacterial Cell Wall Synthesis

    Background:

    • Understanding bacterial cell wall synthesis is crucial for developing new antimicrobial strategies.
    • Previous models suggested localized addition of new cell wall material in some bacteria.

    Purpose of the Study:

    • To investigate the mechanism of cell wall replication in Salmonella typhosa.
    • To compare cell wall synthesis in Salmonella typhosa with other bacterial species.

    Main Methods:

    • Direct labeling of Salmonella typhosa (TY2 W) cell walls with fluorescein-conjugated antibodies.
    • Monitoring fluorescence changes during bacterial growth and in the presence of chloramphenicol.

    Main Results:

    • Cell wall fluorescence decreased uniformly with normal growth and division.
    Keywords:
    CYTOLOGYEXPERIMENTAL LAB STUDYFLUORESCENT ANTIBODY TECHNICSALMONELLA TYPHOSA

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  • Chloramphenicol treatment led to enlarged, non-dividing cells with interrupted wall fluorescence.
  • These observations differ from the localized cell wall addition seen in Streptococcus pyogenes.

    • Conclusions:

    • Salmonella typhosa cell wall synthesis occurs through diffuse intercalation of new material.
    • New cell wall material is formed even when cell division is inhibited by chloramphenicol.