A quantitative chemical study of the comb and wattle galactosaminoglycans from single comb White Leghorn roosters

Galactosaminoglycans from mature rooster comb and wattle tissues were separated into five fractions by ethanol precipitation. An average of 90% total uronic acid was recovered in Fractions I to III. Fractions I and II were dermatan sulfate with relatively high proportions of L-iduronic acid (61 to 80%), but this uronic acid was a minor component (30%) in Fraction III, in which D-glucuronic acid was the major uronic acid. Digestion with testicular hyaluronidase suggested that most if not all of the galactosaminoglycans in Fractions I to III were copolymers containing both L-iduronic acid and D-glucuronic acid. Fractions IV and V contained much lower proportions of L-iduronic acid and showed broader electrophoresis bands than did Fraction III.