Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 14, 2026

Batch Immunostaining for Large-Scale Protein Detection in the Whole Monkey Brain
08:07

Batch Immunostaining for Large-Scale Protein Detection in the Whole Monkey Brain

Published on: July 27, 2009

A novel neutral protease(s) from monkey liver.

K Sogawa, K Takahashi

    Journal of Biochemistry
    |December 1, 1976
    PubMed
    Summary

    Researchers discovered a novel neutral protease in monkey liver. This enzyme, likely membrane-bound, functions optimally at pH 8.0 and is inhibited by DFP and PMSF.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Preferential changes of skeletal muscle echogenicity in myotonic dystrophy type 1.

    European journal of neurology·2016
    Same author

    Involvement of inhibitory PAS domain protein in neuronal cell death in Parkinson's disease.

    Cell death discovery·2016
    Same author

    Inhibitory PAS domain protein is a substrate of PINK1 and Parkin and mediates cell death in a Parkinson's disease model.

    Cell death & disease·2015
    Same author

    Pro-apoptotic activity of inhibitory PAS domain protein (IPAS), a negative regulator of HIF-1, through binding to pro-survival Bcl-2 family proteins.

    Cell death and differentiation·2011
    Same author

    Increased circulating cell signalling phosphoproteins in sera are useful for the detection of pancreatic cancer.

    British journal of cancer·2010
    Same author

    Apolipoprotein C-1 maintains cell survival by preventing from apoptosis in pancreatic cancer cells.

    Oncogene·2007

    Area of Science:

    • Biochemistry
    • Enzymology
    • Molecular Biology

    Background:

    • Proteases play crucial roles in cellular processes.
    • Understanding novel enzymes aids in elucidating biological pathways.
    • Characterizing liver proteases is important for metabolic research.

    Purpose of the Study:

    • To identify and characterize a novel neutral protease from monkey liver.
    • To investigate the enzyme's substrate specificity and optimal conditions.
    • To determine the enzyme's sensitivity to various inhibitors.

    Main Methods:

    • Utilized heat-denatured casein as a substrate.
    • Employed gel filtration chromatography (Ultrogel AcA 22, Sepharose 6B).
    • Solubilized the enzyme using deoxycholate.

    Main Results:

    • A novel neutral protease, presumed membrane-bound, was isolated.
    • Maximal enzyme activity was observed at pH 8.0.
    • The protease was strongly inhibited by diisopropylfluorophosphate (DFP) and phenylmethylsulfonyl fluoride (PMSF).
    • Reagents like TPCK, TLCK, pCMB, iodoacetic acid, and EDTA showed no significant effect.
    • No activation by sodium chloride (NaCl) was observed.

    Conclusions:

    • A unique neutral protease with specific inhibitory profiles was identified in monkey liver.
    • The enzyme's properties suggest a distinct functional role, potentially in membrane-associated processes.
    • Further research is warranted to elucidate the precise biological function and localization of this novel protease.

    More Related Videos

    Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve
    09:13

    Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

    Published on: June 14, 2017

    Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods
    10:40

    Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

    Published on: December 21, 2019

    Related Experiment Videos

    Last Updated: May 14, 2026

    Batch Immunostaining for Large-Scale Protein Detection in the Whole Monkey Brain
    08:07

    Batch Immunostaining for Large-Scale Protein Detection in the Whole Monkey Brain

    Published on: July 27, 2009

    Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve
    09:13

    Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

    Published on: June 14, 2017

    Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods
    10:40

    Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

    Published on: December 21, 2019