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Optimized PCR-based Detection of Mycoplasma
06:01

Optimized PCR-based Detection of Mycoplasma

Published on: June 20, 2011

STORAGE OF MYCOPLASMA STRAINS

W H KELTON

    Journal of Bacteriology
    |March 1, 1964
    PubMed

    Insights

    Mycoplasma strains, including avian, human, and canine types, demonstrate excellent survival after freeze-drying and long-term storage. Optimal preservation for Mycoplasma involves storage at -65 C, avoiding repeated freezing and thawing.

    Area of Science:

    • Microbiology
    • Veterinary Science
    • Bacteriology

    Background:

    • Mycoplasma are significant pathogens in avian, human, and canine species.
    • Maintaining viable Mycoplasma cultures is crucial for research and diagnostics.
    • Understanding optimal storage conditions is essential for long-term preservation of Mycoplasma strains.

    Purpose of the Study:

    • To evaluate the survival rates of various Mycoplasma strains under different storage conditions.
    • To assess the impact of lyophilization, storage temperature, and freeze-thaw cycles on Mycoplasma viability.
    • To compare the stability of different Mycoplasma strains under stress conditions.

    Main Methods:

    • Lyophilization (freeze-drying) of 26 Mycoplasma strains (19 avian, 3 human, 3 canine, 1 saprophyte).
    • Storage of lyophilized strains at -26 C, -65 C, and 5 C for extended periods.
    • Assessment of viability, colony size, and growth rate after storage.
    • Evaluation of resistance to alternate freezing and thawing cycles.

    Main Results:

    • All strains survived lyophilization and were viable for 3-4 years.
    • Storage at -65 C maintained viability and organism integrity for at least 12 months.
    • Storage at -26 C resulted in changes in colony size and growth rate after 10 months.
    • Most strains showed rapid viability loss at 5 C, with significant variation in freeze-thaw resistance.

    Conclusions:

    • Lyophilization is a highly effective method for long-term Mycoplasma strain preservation.
    • Ultra-low temperatures (-65 C) are superior for maintaining Mycoplasma viability and characteristics.
    • Storage temperature and freeze-thaw cycles significantly impact Mycoplasma survival rates, with strain-specific variations observed.
    Keywords:
    CULTURE MEDIAESCHERICHIA COLIEXPERIMENTAL LAB STUDYFREEZE DRYINGMYCOPLASMASTAPHYLOCOCCUS

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