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Fluorescein and fluorescein glucuronide in plasma
L L Knudsen1, T Olsen, F Nielsen-Kudsk
1Department of Ophthalmology, Arhus University Hospital, Denmark.
Acta Ophthalmologica
|August 1, 1992
Summary
A new method simplifies measuring free fluorescein in plasma for blood-ocular barrier evaluation. While fluorescein glucuronide contributes to plasma fluorescence, its impact on vitreous fluorescence is minor.
Area of Science:
- Ophthalmology
- Pharmacokinetics
- Analytical Chemistry
Background:
- Accurate evaluation of the blood-ocular barrier (BOB) relies on quantifying free, unconjugated fluorescein in plasma.
- Existing methods may be complex, necessitating simpler, reliable alternatives.
Purpose of the Study:
- To introduce and validate a novel, straightforward method for determining free fluorescein in plasma.
- To assess the contribution of fluorescein glucuronide to total plasma fluorescence after sodium fluorescein administration.
Main Methods:
- Development of a new method based on total free plasma fluorescence and its free fraction.
- Comparison of the new method with differential spectrofluorophotometry.
- Evaluation of fluorescein glucuronide's contribution using plasma concentration-time curves.
Main Results:
- The new method demonstrated excellent correlation with differential spectrofluorophotometry.
- After 1 hour, fluorescein glucuronide accounted for 8.2% of total free plasma fluorescence.
- By 24 hours, fluorescein glucuronide constituted 18.3% of total free plasma fluorescence.
Conclusions:
- The developed method offers a simple and effective means for measuring free fluorescein in plasma.
- Despite the importance of fluorescein glucuronide in BOB assessment, its contribution to vitreous fluorescence is minimal following intravenous fluorescein administration.