[Studies on secondary derivative differential pulse polarography and its applications]

Secondary derivative differential pulse polarography was developed and used for the quantitative analysis of ribostamycin sulfate, kanamycin and their preparations. By using sodium hydroxide-potassium dihydrogen phosphate solution as the base solution, ribostamycin sulfate and kanamycin showed good peaks at 0.105 V and -0.37 V (vs Ag/AgCl) respectively. The linear relationship of ribostamycin sulfate and kanamycin between concentration and peak height was obtained in the concentration range of 1.8-10.8 x 10(-4) mol/L and 3.1-18.6 x 10(-4) mol/L and the determination limit was 7.2 x 10(-9) mol/L and 4.6 x 10(-8) mol/L respectively. This method is simple, rapid and sensitive. The result is accurate.