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Updated: Sep 14, 2026
Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis
Published on: August 26, 2020
Temperature gradient gel electrophoresis: detection of a single base substitution in the cattle beta-lactoglobulin
M K Tee1, C Moran, F W Nicholas
1Department of Animal Science, University of Sydney, New South Wales, Australia.
Abstract:
An A in equilibrium with G transition in exon III is known to differentiate alleles A and B of the cattle beta-lactoglobulin (BLG) gene. A BLG exon III fragment containing the transition site was amplified by the polymerase chain reaction. Temperature gradient gel electrophoresis (TGGE) was then used to detect this transition and hence to genotype cattle: the AT base-pair in allele A was readily distinguished from the GC base-pair of allele B. TGGE can be used to detect any single base-pair substitution, and thus is a powerful method of detecting genetic variability.
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