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The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity
08:26

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity

Published on: December 14, 2016

ANTIBODY RESPONSE TO IMMUNIZATION BY DIFFERENT ROUTES

S SHULMAN, L HUBLER, E WITEBSKY

    Science (New York, N.Y.)
    |August 21, 1964
    PubMed

    Rabbits were injected with human serum albumin once intravenously or intradermally with this antigen plus complete Freund adjuvant. The hemagglutinating titers of the antiserum and of the heavy and light antibody fractions were followed for several months. Circulation of heavy antibody in the intravenously injected group disappeared in 1 month, but in the intradermally injected group, it rose to a maximum titer, then declined, and then showed a second rise in titer. Light antibody showed increasing and then sustained titers over most of the time period. Heavy antibody failed to precipitate with antigen, even when it showed high hemagglutinating titers. Light antibody, in contrast, gave precipitation even when tested at much lower hemagglutination titers

    Keywords:
    ANTIBODY FORMATIONDIALYSISEXPERIMENTAL LAB STUDYFREUND'S ADJUVANTHEMAGGLUTINATIONIMMUNE SERUMSIMMUNOELECTROPHORESISINJECTIONS, INTRAVENOUSMERCAPTOETHANOLPHARMACOLOGYRABBITSSERUM ALBUMINSPECTROPHOTOMETRYSULFHYDRYL COMPOUNDS

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