Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Alveolar macrophage accessory cell function in bronchial asthma.

V Gant1, M Cluzel, Z Shakoor

  • 1Department of Immunology, UMDS (St. Thomas' Campus), London, United Kingdom.

The American Review of Respiratory Disease
|October 1, 1992
PubMed
Summary

Alveolar macrophages (AM) in asthmatic patients showed variable antigen-presenting cell function compared to blood monocytes. A link between AM function and lymphocytes in bronchoalveolar lavage (BAL) fluid was observed in asthma.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adhesion molecule expression on circulating lymphocytes and monocytes in patients with isolated idiopathic retinal vasculitis.

Ocular immunology and inflammation·2012
Same author

Risks of chest drain insertion.

International journal of clinical practice·2008
Same author

Use of dry powder inhalers in COPD.

International journal of clinical practice·2007
Same author

Non-invasive ventilation at home in chronic obstructive pulmonary disease.

International journal of clinical practice·2007
Same author

Pneumococcal vaccination in asthma.

International journal of clinical practice·2006
Same author

Metalloptysis.

The European respiratory journal·2006

Area of Science:

  • Immunology
  • Respiratory Medicine
  • Cell Biology

Background:

  • Peripheral blood monocytes and alveolar macrophages (AM) are crucial antigen-presenting cells.
  • Asthma involves airway inflammation and immune cell dysregulation.
  • Understanding macrophage function in asthma is key to immune response.

Purpose of the Study:

  • To investigate the antigen-presenting capacity of AM versus blood monocytes in asthmatic and healthy individuals.
  • To explore the relationship between AM accessory cell function and bronchoalveolar lavage (BAL) fluid characteristics.

Main Methods:

  • Recruitment of 11 asthmatic and 11 healthy non-atopic subjects.
  • Isolation of peripheral blood monocytes and AM via bronchoalveolar lavage (BAL).
  • Assay of antigen presentation of recall antigens (PPD, SKSD) to autologous T-cells.

Related Experiment Videos

Main Results:

  • AM accessory cell function was variable in asthmatics, often less effective than blood monocytes.
  • A correlation was found between AM function efficacy for two different antigens (PPD and SKSD).
  • In asthmatics, a correlation existed between BAL fluid lymphocyte percentage and the ratio of AM to monocyte antigen-presenting capability.

Conclusions:

  • Alveolar macrophage antigen-presenting capacity is often impaired in asthma.
  • The presence of lymphocytes in BAL fluid correlates with altered AM function in asthmatics.
  • These findings suggest a link between AM accessory function and airway inflammation in bronchial asthma.