Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Haemoglobin and the red cell membrane.

S R Hollán, J G Szelényi, M Hasitz

    Physiologia Bohemoslovaca
    |January 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Structure and Function of Platelet Glycocalicin.

    Thrombosis and haemostasis·2019
    Same author

    Detection of Activation Antigens on Chronic Lymphocytic Leukaemia Cells.

    Leukemia & lymphoma·2016
    Same author

    Increased in vitro Aggregation of Stored Platelets at Room Temperature in Contrast to 37°C.

    Platelets·2010
    Same author

    Long-term treatment of panic disorder with agoraphobia in private practice.

    Journal of psychiatric practice·2005
    Same author

    Managing antipsychotic-induced tardive dyskinesia.

    Drug safety·1999
    Same author

    Transfusion-associated iron overload.

    Current opinion in hematology·1997

    Haemoglobin is a key component of red blood cell membranes, with its membrane content influenced by calcium levels. Alterations within red blood cells impact membrane structure and immunoglobulin binding.

    Area of Science:

    • Hematology
    • Biochemistry
    • Cell Biology

    Background:

    • Red blood cell (RBC) membrane integrity is crucial for its function.
    • The interaction between intracellular components and the RBC membrane is not fully understood.
    • Haemoglobin's role within the RBC membrane requires further elucidation.

    Purpose of the Study:

    • To investigate the integral role of haemoglobin within the red blood cell membrane.
    • To determine the influence of calcium (Ca++) on haemoglobin content in the RBC membrane.
    • To explore how intracellular changes affect RBC membrane organization and surface interactions.

    Main Methods:

    • Analysis of haemoglobin content in isolated red blood cell membranes.
    • Assessment of calcium levels within the red blood cell membrane.

    Related Experiment Videos

  • Evaluation of immunoglobulin binding to the red blood cell surface following induced intracellular changes.
  • Comparative analysis of haemoglobin variants (Hb-s A2, Hb S, Hb A) binding to the membrane.

    • Main Results:

    • Haemoglobin is confirmed as an integral component of the red blood cell membrane.
    • Membrane haemoglobin content shows a strong correlation with membrane calcium (Ca++) levels in both healthy and diseased states.
    • Intracellular alterations significantly modify RBC membrane organization and immunoglobulin binding patterns.
    • Preferential binding of haemoglobin S (Hb S) and haemoglobin A2 (Hb A2) to the membrane was observed, independent of charge differences compared to haemoglobin A (Hb A).

    Conclusions:

    • Haemoglobin plays a structural role within the red blood cell membrane.
    • Calcium ions are critical regulators of haemoglobin association with the RBC membrane.
    • Red blood cell internal environment dynamics directly influence membrane structure and function, including immune interactions.
    • Specific haemoglobin variants exhibit distinct membrane-binding properties that are not solely charge-dependent.