Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ascorbic acid biosensor using ascorbate oxidase immobilized on alkylamine glass beads.

E T Marques1, J L Lima Filho

  • 1Departamento de Bioquimica, Universidade Federal de Pernambuco, Brazil.

Applied Biochemistry and Biotechnology
|January 11, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Establishment and cryptic transmission of Zika virus in Brazil and the Americas.

Nature·2017
Same author

Can artisanal "Coalho" cheese from Northeastern Brazil be used as a functional food?

Food chemistry·2012
Same author

Immune transcript variations among Aedes aegypti populations with distinct susceptibility to dengue virus serotype 2.

Acta tropica·2012
Same author

Production of lipase free of citrinin by Penicillium citrinum.

Mycopathologia·2010
Same author

The sound of silence: human beta-defensin-1 gene untranslated SNPs change the predicted mRNA secondary structure in a length-dependent manner.

Immunology letters·2010
Same author

HPV31 and HPV33 incidence in cervical samples from women in Recife, Brazil.

Genetics and molecular research : GMR·2009

A new biosensor for ascorbic acid was developed using ascorbate oxidase enzyme immobilized on glass beads. This low-cost system demonstrates good sensitivity and operational stability for ascorbic acid detection.

Area of Science:

  • Biochemistry
  • Biosensor Technology
  • Enzyme Kinetics

Background:

  • Ascorbic acid (Vitamin C) is vital for human health.
  • Accurate measurement of ascorbic acid is crucial in various fields.
  • Existing methods for ascorbic acid detection can be complex or costly.

Purpose of the Study:

  • To develop a novel, cost-effective biosensor for ascorbic acid detection.
  • To utilize the enzyme kinetics of ascorbate oxidase for biosensor development.
  • To immobilize ascorbate oxidase effectively for enhanced biosensor performance.

Main Methods:

  • Ascorbate oxidase was extracted from Cucurbita maxima (jerimun).
  • Enzyme immobilization was achieved via covalent bonding using glutaraldehyde on alkylamine glass beads.

Related Experiment Videos

  • A homemade oxygen electrode served as the transducer.
  • Enzyme active site protection during immobilization was investigated.

    • Main Results:

    • The developed biosensor showed sensitivity for ascorbic acid concentrations ranging from 62.5 to 500 microM.
    • The biosensor maintained satisfactory operation for over 2 months.
    • Immobilization strategies, with and without active site protection, were evaluated.

    Conclusions:

    • A functional and stable biosensor for ascorbic acid detection was successfully developed.
    • The biosensor offers a low-cost alternative for ascorbic acid measurement.
    • The enzyme kinetics of immobilized ascorbate oxidase provide a reliable basis for biosensing applications.