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RESPONSE OF CLOSTRIDIUM PERFRINGENS SPORES AND VEGETATIVE CELLS TO TEMPERATURE VARIATION.
Applied Microbiology
|May 1, 1964
Summary
Clostridium perfringens vegetative cells and spores show differing survival rates at extreme temperatures. Spores exhibit greater resistance to cold and heat compared to vegetative cells.
Area of Science:
- Microbiology
- Food Safety
Background:
- Clostridium perfringens is a significant foodborne pathogen.
- Understanding the thermal resistance of its vegetative cells and spores is crucial for food safety.
Purpose of the Study:
- To investigate the survival of Clostridium perfringens vegetative cells and spores under various temperature conditions.
- To compare the thermal tolerance between vegetative cells and spores of different strains.
Main Methods:
- Vegetative cells were cultured in thioglycolate broth.
- Spores were produced using Ellner's method.
- Cultures were exposed to freezing (-17.7 C), refrigeration (7.1 C), and elevated temperatures (80 C for 10 min).
Main Results:
- Low numbers of vegetative cells survived freezing and refrigeration.
- 16-58% of spores survived freezing and refrigeration.
- Minimal survival of both vegetative cells and spores was observed at 80 C for 10 minutes.
Conclusions:
- Clostridium perfringens spores are significantly more resistant to thermal stress than vegetative cells.
- Temperature control is critical in preventing the survival and proliferation of Clostridium perfringens in food products.