Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Isolated biventricular working rat heart preparation.

T L Demmy1, G J Magovern, R L Kao

  • 1Department of Surgery, Medical College of Pennsylvania, Pittsburgh, Pennsylvania.

The Annals of Thoracic Surgery
|November 1, 1992
PubMed
Summary

A new biventricular working heart preparation allows for comprehensive study of isolated rat hearts. This model enhances functional evaluations compared to previous methods, enabling better research into total heart performance.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcription factor Phox2 upregulates expression of norepinephrine transporter and dopamine β-hydroxylase in adult rat brains.

Neuroscience·2011
Same author

Overexpression of vascular endothelial growth factor 165 (VEGF165) protects cardiomyocytes against doxorubicin-induced apoptosis.

Journal of chemotherapy (Florence, Italy)·2011
Same author

Management of locoregional stage esophageal cancer: a single center experience.

Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus·2006
Same author

Tracheostomy: a risk factor for mediastinitis after cardiac operation.

The Annals of thoracic surgery·2001
Same author

Laboratory confirmation of clinical heart allograft preservation variability.

The Annals of thoracic surgery·2001
Same author

Illness representations according to age and effects on health behaviors following coronary artery bypass graft surgery.

Journal of the American Geriatrics Society·2001

Area of Science:

  • Cardiovascular Physiology
  • Experimental Cardiology
  • Animal Models

Background:

  • Isolated perfused heart models are crucial for hemodynamic and metabolic research.
  • The left working heart preparation is effective but limited to left heart function.
  • A need exists for a model that assesses both left and right heart function simultaneously.

Purpose of the Study:

  • To develop a simple, cost-effective biventricular working heart preparation for isolated rat hearts.
  • To enable simultaneous functional evaluation of both ventricles in a perfused heart model.
  • To overcome the limitations of the traditional left working heart preparation.

Main Methods:

  • Modification of the left working rat heart model to include biventricular cannulation.

Related Experiment Videos

  • Surgical removal of the heart under general anesthesia with attached major vessels.
  • Utilized a pressurized compliance chamber for precise aortic impedance control.
  • Performed 3-hour perfusions to assess hemodynamic and metabolic parameters.

    • Main Results:

    • The biventricular model maintained physiologic levels of aortic output, pulmonary flow, pressures, and heart rate for 3 hours.
    • Myocardial adenosine triphosphate and creatine phosphate levels remained stable, indicating good metabolic support.
    • Conversion from left to biventricular mode showed significant improvements in stroke volume and cardiac output.
    • The biventricular model demonstrated superior performance for assessing total heart function.

    Conclusions:

    • The developed biventricular working heart preparation is a superior model for studying the integrated function of the entire rat heart.
    • This model overcomes the limitations of the left-only working heart preparation.
    • Further research utilizing this advanced biventricular model is recommended for comprehensive cardiovascular studies.