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Updated: Jun 24, 2026

Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana
Published on: August 17, 2009
ENDIVE PLANTLETS FROM FREELY SUSPENDED CELLS AND CELL GROUPS GROWN IN VITRO
Abstract:
Callus tissue derived from mature embryos of the endive, Cichorium endivia Linn. (family Compositae) grows and develops chlorophyll on a completely defined nutrient medium. The tissue breaks up into a thick suspentsion of cells and cell groups in a liquid medium kept in a flask on a shaker. Gradually, many small round masses of tissue, designated here as embryoids, are formed; these become differentiated and organized to form numnerous small plantlets having typical curled and fringed green leaves and roots.
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