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Production of a low molecular weight growth inhibitory factor by adenovirus 12-transformed cells
1Department of Biology, Faculty of Science, Niigata University, Japan.
Abstract:
Malignant rodent cells transformed by human adenovirus 12 produce a potent cell growth inhibitory factor. The cell growth inhibitory factor inhibits the growth of and DNA synthesis in normal fibroblasts in vitro. Extent of the production of the cell growth inhibitory factor appears to be proportional to that of the malignancy of the transformed cells. C57AT1-AB cells, an adenovirus 12-transformant of C57BL/6 mouse origin, are highly tumorigenic in the syngeneic and allogeneic mice. The cell growth inhibitory factor produced by these cells was characterized for the physicochemical properties; the cell growth-inhibitory activity was quantitatively recovered in the filtrates of YM-2 membrane (M(r) less than 1,000), resistant to the heat treatments at 56 degrees C for 30 min and 100 degrees C for 5 min, and extractable by ethyl acetate under acid-condition. These results suggest that the cell growth inhibitory factor may be lipid or oligopeptides.
Insights
Malignant cells infected with human adenovirus 12 release a factor that inhibits normal cell growth and DNA synthesis. This cell growth inhibitory factor
Area of Science:
- Oncology
- Virology
- Molecular Biology
Background:
- Human adenovirus 12 (Ad12) can transform rodent cells, leading to malignancy.
- Transformed cells may produce factors influencing tumor growth and host interactions.
Purpose of the Study:
- To investigate the production and characteristics of a cell growth inhibitory factor from Ad12-transformed malignant rodent cells.
- To determine the physicochemical properties of this inhibitory factor.
Main Methods:
- Characterization of cell-free filtrates from Ad12-transformed C57AT1-AB cells.
- Assay of cell growth and DNA synthesis inhibition in normal fibroblasts.
- Physicochemical analyses including membrane filtration, heat stability, and solvent extraction.
Main Results:
- Ad12-transformed malignant cells produced a potent cell growth inhibitory factor.
- Inhibition of normal fibroblast growth and DNA synthesis was observed.
- The factor has a molecular weight less than 1,000, is heat-resistant, and extractable by ethyl acetate under acidic conditions.
Conclusions:
- The cell growth inhibitory factor's production correlates with cellular malignancy.
- The factor's properties suggest it may be a lipid or oligopeptide.
- Further characterization is needed to elucidate the exact nature of this tumor-associated factor.