Isolation of streptococcal nuclease B by batch adsorption

Area of Science:

• Biochemistry
• Enzymology
• Immunology

Background:

• Streptococcal nuclease B is an enzyme relevant to immune response.
• Accurate measurement of anti-deoxyribonuclease B antibodies requires a well-characterized antigen.

Purpose of the Study:

• To develop a purification method for streptococcal nuclease B.
• To assess the suitability of the purified enzyme as an antigen for antibody detection.

Main Methods:

• Enzyme preparation utilizing batch adsorption.
• Purification chromatography on diethylaminoethyl-cellulose.

Main Results:

• A method for preparing streptococcal nuclease B was established.
• The resulting enzyme preparation demonstrated homogeneity in nuclease activity.
• The purified enzyme is suitable for use as an antigen.

Conclusions:

• The developed method provides a homogeneous streptococcal nuclease B preparation.
• This purified enzyme is effective for quantifying anti-deoxyribonuclease B antibody levels in sera.