Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Platelet-activating factor antagonists in experimental shock.

G Muacevic¹, H O Heuer

¹Department of Pharmacology, Boehringer Ingelheim KG, Ingelheim/Rhein, Fed. Rep. of Germany.

Arzneimittel-Forschung

|August 1, 1992

Summary

Platelet-activating factor (PAF) causes significant physiological changes in rats, mimicking endotoxin shock. PAF antagonists effectively reversed these effects, highlighting their therapeutic potential.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Comparative effects of a glucocorticosteroid, theophylline and the peptido-leukotriene-antagonist CGP 45715A on antigen-induced early and late phase airway response and inflammatory cell influx in sensitised guinea pigs.

European journal of pharmacology·1999

Same author

Characterisation of a novel airway late phase model in the sensitized guinea pig which uses silica and Bordetella pertussis as adjuvant for sensitization.

European journal of pharmacology·1996

Same author

Pharmacological characterisation of a new model of antigen-induced pulmonary late-phase reaction in the conscious guinea pig which uses additional polymyxin B inhalation.

Journal of lipid mediators and cell signalling·1996

Same author

Pulmonary pharmacology of DT-TX 30 SE, a potent selective combined thromboxane synthetase inhibitor and receptor antagonist, in guinea pigs.

Japanese journal of pharmacology·1996

Same author

Dissociation of airway responsiveness and bronchoalveolar lavage (BAL) cell composition in sensitized guinea-pigs after daily inhalation of ovalbumin.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·1994

Same author

Current status of PAF antagonists.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·1992

Area of Science:

Pharmacology
Physiology
Toxicology

Background:

Platelet-activating factor (PAF) is a potent mediator implicated in various physiological responses.
Endotoxin shock involves complex pathophysiological mechanisms, including hemodynamic and metabolic alterations.

Purpose of the Study:

To investigate the physiological effects of PAF in rats.
To evaluate the efficacy of selective hetrazepinoic PAF antagonists in counteracting PAF-induced changes and mimicking endotoxin shock.
To clarify the role of PAF in endotoxin shock models.

Main Methods:

Administration of Platelet-activating factor (PAF) intravenously to rats at specific dosages.
Induction of hypotension using PAF infusions or intravenous injection of E. coli endotoxin.

Related Experiment Videos

Treatment with selective hetrazepinoic PAF antagonists: apafant (WEB 2086), bepafant (WEB 2170), and STY 2108, both pre- and post-treatment.

Monitoring of hematological parameters, ECG, acid-base balance, and blood pressure.

Main Results:

Intravenous PAF induced hyperkalemia, altered hematological parameters, ECG changes, and acid-base imbalance in rats.
PAF administration and E. coli endotoxin caused a significant drop in blood pressure.
Pre- and post-treatment with apafant, bepafant, and STY 2108 effectively antagonized the observed PAF-induced and endotoxin-induced effects.

Conclusions:

Platelet-activating factor (PAF) can replicate key features of endotoxin shock in a rat model.
Selective hetrazepinoic PAF antagonists demonstrate significant efficacy in reversing PAF-mediated physiological disturbances.
These findings underscore the utility of hetrazepines in elucidating the role of PAF in shock conditions.