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New analogues of Cucurbita maxima trypsin inhibitor III (CMTI III) with simplified structure
K Rolka1, G Kupryszewski, J Rózycki
1Faculty of Chemistry, University of Gdańsk, Poland.
Biological Chemistry Hoppe-Seyler
|October 1, 1992
Abstract:
Seven new analogues of trypsin inhibitor CMTI III were obtained by solid-phase peptide synthesis. Three analogues contained only two, instead of three, disulfide bridges, whereas the molecules of the next four analogues were shortened at the N- and/or C-terminus. The elimination of one disulfide bridge in CMTI III induces a decrease in the association equilibrium constants by 6-7 orders of magnitude, whereas the removal of one, two or three amino-acid residues at the N- and/or C-terminus does not significantly affect the activity.