PLAQUE ASSAY FOR PSITTACOSIS VIRUS IN MONOLAYERS OF CHICK EMBRYO FIBROBLASTS
Abstract:
Piraino, F. (City of Milwaukee Health Department, Milwaukee, Wis.), and C. Abel. Plaque assay for psittacosis virus in monolayers of chick embryo fibroblasts. J. Bacteriol. 87:1503-1511. 1964.-A tissue culture system for the assay of 6BC psittacosis virus by plaque production on chick embryo fibroblast cells is described. Experiments are given which characterize the specificity of the reaction and identify factors found to cause variations in plaque counts. The statistical data obtained indicate this assay to be a precise and highly reliable procedure for the detection of 6BC virus and antibody.
Insights
A new plaque assay method using chick embryo fibroblast cells precisely detects psittacosis virus (6BC) and antibodies. This reliable tissue culture system offers accurate quantification for diagnostic purposes.
Area of Science:
- Microbiology
- Virology
- Cell Biology
Background:
- Psittacosis virus, a significant pathogen, requires accurate detection methods.
- Existing diagnostic techniques may lack precision or require specialized equipment.
Purpose of the Study:
- To describe a novel plaque assay for quantifying psittacosis virus (6BC).
- To establish a reliable tissue culture system for virus and antibody detection.
Main Methods:
- Utilized chick embryo fibroblast cell monolayers for plaque formation.
- Performed experiments to characterize reaction specificity and identify factors affecting plaque counts.
Main Results:
- Developed a precise and highly reliable plaque assay for 6BC psittacosis virus.
- Identified key factors influencing plaque count variations.
Conclusions:
- The described plaque assay is a valuable tool for accurate psittacosis virus detection.
- This method provides a reliable basis for diagnosing psittacosis infections and identifying antibodies.
