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Updated: Aug 18, 2026

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
Published on: December 1, 2017
A rapid and reliable method to create tandem arrays of short DNA sequences
1Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461.
Abstract:
Tandemly polymerized regulatory elements, antisense RNA segments or ribozymes are potentially useful in selective gene silencing. However, existing methods of tandemly polymerizing short DNA segments are laborious. We present a procedure that can create cloned arrays of 40-70 monomer units in two steps. We have created long arrays of regulatory elements and potential ribozyme sequences. Silencing of human immunodeficiency virus (HIV-1) activation by tandem arrays of a regulatory element in human immune system cells and in other human and monkey cells is discussed.
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