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Updated: Jul 28, 2026
08:53
Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma
Published on: June 10, 2017
p53 expression in Reed-Sternberg cells of Hodgkin's disease
R K Gupta1, A J Norton, I W Thompson
1ICRF Department of Medical Oncology, St Bartholomew's Hospital, West Smithfield, London, UK.
British Journal of Cancer
|October 1, 1992
Summary
Abnormal p53 protein expression is common in cancers. This study found abnormal p53 in most Hodgkin
Area of Science:
- Oncology
- Molecular Biology
- Immunohistochemistry
Background:
- Mutation of the p53 protein is a frequent genetic event in human cancers.
- Abnormal p53 expression is documented in various malignancies, but its role in Hodgkin's disease is less understood.
Purpose of the Study:
- To investigate the expression of the p53 nuclear phosphoprotein in Hodgkin's disease.
- To determine if p53 alterations correlate with specific subtypes or disease progression.
Main Methods:
- Immunohistochemical analysis of p53 expression using polyclonal (CM-1) and monoclonal (PAb 1801, PAb 240) antibodies.
- Examination of paraffin-embedded biopsies from 50 Hodgkin's disease patients and snap-frozen material.
- Assessment of serial biopsies to evaluate p53 expression changes upon disease recurrence.
Main Results:
- Abnormal p53 immunoreactivity was detected in Reed-Sternberg cells and mononuclear variants in nodular sclerosing (86%) and mixed cellularity (57%) Hodgkin's disease subtypes.
- No positive p53 staining was observed in the nodular lymphocyte predominant type of Hodgkin's disease.
- Consistent p53 expression patterns were noted in serial biopsies following disease recurrence.
Conclusions:
- Overexpression of p53, likely in a mutant form, is implicated in the tumorigenesis of Hodgkin's disease.
- p53 alterations may serve as a potential biomarker or therapeutic target in specific Hodgkin's disease subtypes.
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