Related Experiment Video
Updated: Jul 14, 2026
Culturing Primary Rat Inner Medullary Collecting Duct Cells
Published on: June 21, 2013
Primary structure of rat renal dipeptidase and expression of its mRNA in rat tissues and COS-1 cells
H Adachi1, N Ishida, M Tsujimoto
1Suntory Institute for Biomedical Research, Osaka, Japan.
Abstract:
Three cDNA clones of rat renal dipeptidase (rrDP) were isolated from rat renal and pulmonary cDNA libraries using a DNA fragment of human renal DP cDNA clone, MDP4, as a probe. The complete amino acid sequence deduced from the cDNA contains 410 amino acid residues, beginning with a signal peptide of 16 amino acid residues. RNA blot hybridization analysis showed that 1.6 and 2.2 kb mRNAs were expressed in lung and kidney, however, only 1.6 kb mRNA was detected in small intestine. COS-1 cells transfected with the cDNA expressed enzymatically active rrDP.
