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Preparation of 5- and 6-(aminomethyl)fluorescein
1Abbott Laboratories, Diagnostics Division, Abbott Park, Illinois 60064.
Bioconjugate Chemistry
|September 1, 1992
Abstract:
5(6)-Carboxyfluorescein is protected as the diacetate then reduced to 5(6)-(hydroxymethyl)fluorescein diacetate. The separated isomers are subjected to a Mitsunobu reaction with dibenzyl imidodicarbonate, yielding diprotected 5- and 6-(aminomethyl)fluorescein diacetate. Methanolysis of the acetates followed by deprotection with HBr/acetic acid gives 5- and 6-(aminomethyl)fluorescein hydrobromide.