Related Experiment Videos
Determination of thevetin B in serum by fluorescence polarization immunoassay
E Uber-Bucek1, M Hamon, C Pham Huy
1Laboratoire de Pharmacognosie, Faculté des Sciences, Chatenay Malabry, France.
Journal of Pharmaceutical and Biomedical Analysis
|June 1, 1992
Abstract:
Thevetin B, a cardiac glycoside of Thevetia neriifolia Juss. seeds, was determined in serum by fluorescence polarization immunoassay. Anti-digitoxin antibody was used, thevetin B genin being structurally identical to digitoxigenin. Cross-reactivity of 94% was found by this method, for concentrations from 5 to 80 ng ml-1.