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Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples
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Enrichment and Detection of Clostridium perfringens Toxinotypes in Retail Food Samples

Published on: October 18, 2019

ISOLATION OF TOXIGENIC STRAINS OF CLOSTRIDIUM PERFRINGENS FROM SOIL

T YAMAGISHI, S ISHIDA, S NISHIDA

    Journal of Bacteriology
    |September 1, 1964
    PubMed

    Insights

    Toxigenic Clostridium perfringens strains were isolated from soil. Higher heating temperatures reduced toxigenic strain isolation, suggesting sporulating ability is linked to Clostridium perfringens toxigenicity.

    Area of Science:

    • Microbiology
    • Bacteriology

    Background:

    • Clostridium perfringens is an important bacterial species.
    • Understanding the factors influencing its toxigenicity is crucial.

    Purpose of the Study:

    • To isolate toxigenic strains of Clostridium perfringens from soil.
    • To investigate the relationship between heat resistance and toxigenicity.

    Main Methods:

    • Soil specimens were subjected to various heating temperatures and durations.
    • Isolated strains were toxigenically examined.
    • Heat resistance of toxigenic and non-toxigenic strains was assessed.

    Main Results:

    • Fewer toxigenic Clostridium perfringens strains were isolated at higher temperatures.
    • No toxigenic strains were recovered from soil heated at 100°C for 60 minutes.
    • All toxigenic strains were killed by heating at 100°C for 10 minutes; resistant strains were non-toxigenic.

    Conclusions:

    • Sporulating ability appears to be closely linked to the toxigenicity of Clostridium perfringens.
    • Heat resistance can be an indicator of non-toxigenicity in Clostridium perfringens.
    Keywords:
    BIOLOGICAL ASSAYCLOSTRIDIUM PERFRINGENSEXPERIMENTAL LAB STUDYSOIL MICROBIOLOGYSPORESTEMPERATURETOXICOLOGIC REPORTTOXINS AND ANTITOXINS

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