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UPTAKE OF VALINE AND GLYCYLVALINE BY LEUCONOSTOC MESENTEROIDES
Journal of Bacteriology
|October 1, 1964
Summary
Leuconostoc mesenteroides utilizes distinct, energy-dependent systems for valine and glycylvaline uptake. Glycylvaline supports faster growth, but other dipeptides can interfere with its utilization.
Area of Science:
- Microbiology
- Biochemistry
- Cell Biology
Background:
- Amino acid and peptide transport are crucial for bacterial growth.
- Leuconostoc mesenteroides is a lactic acid bacterium with known metabolic capabilities.
Purpose of the Study:
- To investigate the uptake mechanisms of valine and glycylvaline in Leuconostoc mesenteroides.
- To determine if separate transport systems exist for amino acids and dipeptides.
Main Methods:
- Studied uptake in growing cultures and non-growing cellular suspensions.
- Utilized radiolabeled (C14) and unlabeled amino acids and peptides.
- Performed chromatographic identification of synthesized glycyl-dl-valine-1-C14.
Main Results:
- Identified distinct, energy-dependent, and saturable uptake systems for valine and glycylvaline.
- Observed that glycylvaline promotes more rapid growth than valine.
- Demonstrated antagonism of other dipeptides towards glycylvaline utilization.
Conclusions:
- Leuconostoc mesenteroides possesses specific transport systems for both valine and glycylvaline.
- Dipeptide uptake is an active process that can be influenced by other peptides.