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Improved resolution in three-dimensional constant-time triple resonance NMR spectroscopy of proteins
A G Palmer1, W J Fairbrother, J Cavanagh
1Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037.
Journal of Biomolecular NMR
|January 1, 1992
Abstract:
Two new protocols for the three-dimensional, triple resonance, constant-time HCA(CO)N NMR experiment are presented that significantly increase the experimental resolution attainable in the C alpha frequency dimension. Experimental verification of the new experiments is provided by spectra of the IIA domain of glucose permease from Bacillus subtilis.