Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Isolated hyperthermic liver perfusion with cytostatic-containing perfusate activates the complement cascade.

J P Arnestad1, A Bengtsson, J P Bengtson

  • 1Department of Anaesthesiology and Intensive Care, Sahlgren's Hospital, Gothenburg, Sweden.

The British Journal of Surgery
|September 1, 1992
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Observation of disorder-free localization using a (2+1)D lattice gauge theory on a quantum processor.

Science (New York, N.Y.)·2026
Same author

Clinical and histopathological features of a population-based cohort of 95 consecutive patients with immunoglobulin G4-related disease in Stockholm, Sweden, during a five-year period.

Scandinavian journal of rheumatology·2026
Same author

Analysis of negligence claims after carotid artery interventions over 20 years in Sweden.

Scandinavian journal of surgery : SJS : official organ for the Finnish Surgical Society and the Scandinavian Surgical Society·2025
Same author

Visualizing dynamics of charges and strings in (2 + 1)D lattice gauge theories.

Nature·2025
Same author

Scaling and logic in the colour code on a superconducting quantum processor.

Nature·2025
Same author

Thermalization and criticality on an analogue-digital quantum simulator.

Nature·2025

This study investigated complement activation during liver perfusion therapy for advanced liver malignancy. Melphalan and cisplatin treatments activated complement, particularly at higher temperatures, indicating a potential mechanism for side effects.

Area of Science:

  • Immunology
  • Oncology
  • Pharmacology

Background:

  • Isolated hyperthermic liver perfusion (IHLP) is used for advanced liver malignancy.
  • The study examines complement activation during IHLP with melphalan and cisplatin.

Purpose of the Study:

  • To investigate complement activation, including anaphylatoxins (C3a, C5a) and terminal C5b-9 complement complexes (TCCs), during IHLP.
  • To determine if melphalan and cisplatin activate complement in vitro and if temperature influences this activation.

Main Methods:

  • Blood and perfusate samples were collected from eight patients undergoing IHLP.
  • Complement variables (C1-INH, C3, C4, C5, C3a, C5a, TCCs) were measured at various time points.
  • In vitro experiments assessed melphalan and cisplatin's effect on complement activation at 37°C and 42°C.

Related Experiment Videos

Main Results:

  • Complement activation was observed during IHLP.
  • Elevated plasma concentrations of C3a and TCCs were detected post-perfusion.
  • High levels of C3a and TCCs were found in the perfusate.
  • Melphalan and cisplatin demonstrated complement activation in vitro, more pronounced at 42°C.

Conclusions:

  • IHLP with melphalan and cisplatin triggers complement activation in patients with advanced liver malignancy.
  • The chemotherapeutic agents may directly activate complement, with temperature as a contributing factor.
  • Findings suggest a potential link between complement activation and treatment-related adverse events.