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Hemopoietic functions of marrow-derived osteogenic cells
D Benayahu1, M Horowitz, D Zipori
1Department of Histology and Cell Biology, Sackler Faculty of Medicine, Tel-Aviv University, Ramat-Aviv, Israel.
Calcified Tissue International
|September 1, 1992
Summary
Marrow osteogenic cells (MBA-15) produce cytokines that regulate blood cell growth. These cells can both stimulate and inhibit myeloid and lymphoid cell development, impacting hematopoiesis.
Area of Science:
- Hematology
- Cell Biology
- Bone Biology
Background:
- Osteoblasts are part of the marrow stromal network involved in bone remodeling.
- Their role in the hemopoietic microenvironment is actively investigated.
- Marrow-derived osteogenic cells (MBA-15) exhibit osteoblastic characteristics.
Purpose of the Study:
- To investigate the stromal functions of marrow-derived osteogenic cells (MBA-15).
- To determine if MBA-15 cells produce cytokines that influence hemopoiesis.
- To explore the mechanisms of interaction between MBA-15 cells and hematopoietic cells.
Main Methods:
- Characterization of MBA-15 cells in vitro and in vivo.
- Detection and identification of secreted cytokines (M-CSF, GM-CSF, IL-6).
- Co-culture experiments with MBA-15 cells and various myeloid and lymphoid cells.
Main Results:
- MBA-15 cells constitutively produce and secrete M-CSF, GM-CSF, and IL-6.
- MBA-15 cells modulate the growth of normal and malignant myeloid and lymphoid cells.
- Both soluble and membrane-bound inhibitory activities from MBA-15 cells affect hematopoietic cell growth.
Conclusions:
- Marrow osteogenic cells (MBA-15) possess significant stromal functions.
- These cells produce a balance of stimulatory and inhibitory factors affecting hematopoiesis.
- Osteogenic cells likely play a regulatory role in the bone marrow's hematopoietic microenvironment.