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Related Experiment Videos

Intrasplenic islet isografts.

S D Feldman, G E Hirshberg, G Dodi

    Surgery
    |September 1, 1977
    PubMed
    Summary
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    Transplanting pancreatic islets into the spleen reverses diabetes in rats but impairs insulin response. Most transplanted islets may lodge in the liver, not the spleen.

    Area of Science:

    • Endocrinology
    • Transplantation Biology
    • Diabetology

    Background:

    • Streptozotocin-induced diabetes in rats is a model for type 1 diabetes.
    • Pancreatic islet transplantation is a potential therapy for diabetes.
    • The splenic pulp is a common site for islet transplantation.

    Purpose of the Study:

    • To evaluate the efficacy of intrasplenic pancreatic islet transplantation in diabetic rats.
    • To assess the insulin response and tissue distribution after intrasplenic islet transplantation.

    Main Methods:

    • Induction of diabetes in rats using streptozotocin.
    • Transplantation of pancreatic islets into the splenic pulp.
    • Intravenous glucose tolerance tests (IVGTT) to assess insulin response.

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  • Splenectomy to determine tissue distribution.

    • Main Results:

    • Intrasplenic islet transplantation reversed hyperglycemia, glycosuria, and polyuria, promoting weight gain for at least 3 months.
    • Animals showed impaired biphasic insulin response during IVGTT compared to portal vein-transplanted animals.
    • Basal serum insulin levels were elevated in intrasplenic recipients.
    • Splenectomy indicated that a significant portion of transplanted islets lodged in organs other than the spleen, primarily the liver.

    Conclusions:

    • Intrasplenic pancreatic islet transplantation can achieve functional diabetes reversal in rats.
    • The impaired insulin response suggests altered islet function or hepatic metabolism.
    • Hepatic sequestration of islets is a significant factor influencing transplant outcomes.