Large serotonin-like immunoreactive amacrine cells in the retina of developing Xenopus laevis

The earliest appearance of serotonin-like immunoreactivity (SLI) in different cell types and the development of large SLI amacrine cells were studied in the retina of Xenopus laevis from stage 33/34 to adult. Intense SLI was first found in the somas of large amacrine cells at stage 39. The somas of small amacrine cells showed weak SLI at stage 41, followed by bipolar cells at stage 43. The number of large SLI amacrine cells in the inner nuclear layer of the retina increased from 57 at stage 40 to 774 in adult. Over the same period, retinal area increased from 0.19 mm2 to 24.57 mm2 with an accompanying decrease of cell density from 301/mm2 to 32/mm2. in adult animals large SLI amacrine cells were non-uniformly distributed. Peak cell density of 50-60/mm2 was located in the center of the ventrotemporal quadrant and a trough of 8-15/mm2 in the dorsal periphery of the retina. Peak cell density region of the adult retina corresponded to part of the retina formed at early developmental stages where the rate of cell generation of large SLI amacrine cells was higher. These observations indicate that (1) SLI is expressed first by large amacrine cells, followed by small amacrine and bipolar cells; (2) large SLI amacrine cells are generated continuously throughout life, (3) the non-uniform retinal distribution of large cells results from a spatio-temporally differential cell generation at the ciliary margin.