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Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
06:29

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Published on: January 29, 2014

COMPLEMENT AND HEMOLYTIC ANTIBODY: CHANGES IN THEIR ACTIVITY INDUCED BY MERCAPTOETHANOL

M M FRANK, T BORSOS, H J RAPP

    Science (New York, N.Y.)
    |February 12, 1965
    PubMed

    Cell-bound complement components are not destroyed by mercaptoethanol. Destruction of cell-bound hemolytic antibody was prevented by cell-bound complement components. In the absence of cells, the complement components C'3 and C'4 were most susceptible to destruction by mercaptoethanol; C'1, C'1a, and C'2 were most resistant. Mercaptoethanol (0.01M) increases C'1 activity, and this increase is not due to activation of C'1 to C'1a. Destruction of some components required both mercaptoethanol and iodoacetamide treatment.

    Keywords:
    ANTIBODIESCENTRIFUGATIONCOMPLEMENTDIALYSISEXPERIMENTAL LAB STUDYGUINEA PIGSHEMOLYSISMERCAPTOETHANOLPHARMACOLOGYRABBITSSHEEPSULFHYDRYL COMPOUNDS

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