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A cDNA clone for a novel nuclear protein with DNA binding activity
T Mattioni1, C R Hume, S Konigorski
1Immunology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021.
Chromosoma
|October 1, 1992
Summary
Researchers identified a new human gene, DBP-5, which is found in the nucleus and binds DNA. This discovery suggests DBP-5 may be involved in chromatin organization and gene regulation.
Area of Science:
- Molecular Biology
- Genetics
- Cell Biology
Background:
- Identifying trans-acting factors is crucial for understanding gene regulation.
- Novel gene discovery is essential for expanding our knowledge of cellular mechanisms.
Purpose of the Study:
- To clone and characterize a novel human gene involved in gene regulation.
- To investigate the potential role of the DBP-5 gene in cellular processes.
Main Methods:
- Gene cloning and cDNA sequencing.
- mRNA distribution analysis.
- Immunofluorescence for protein localization.
- DNA binding assays.
Main Results:
- A novel human gene, DBP-5, was cloned, encoding a 1,179 amino acid protein.
- DBP-5 mRNA is ubiquitously distributed and the gene is phylogenetically conserved.
- The DBP-5 protein localizes to the nucleus and exhibits DNA-binding capabilities.
Conclusions:
- The DBP-5 gene is a conserved, ubiquitously expressed nuclear protein with DNA-binding activity.
- DBP-5 may play a significant role in interphase chromatin organization and/or transcriptional regulation.