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Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
Published on: September 27, 2013
The action of interleukin 6 on lymphoid populations
1Central Laboratory of the Netherlands Red Cross Bloodtransfusion Service, Amsterdam.
Abstract:
We have analysed the role of IL-6 in activation of human lymphocytes isolated from peripheral blood. We found little effect of IL-6 on proliferation of T cells. IL-6 did stimulate production of IgM by B cells. However, it did not behave as a terminal differentiation factor and was required only during the first two days of the culture. Under most conventional stimulation conditions T cells themselves produce little or no IL-6. High IL-6 production could be induced by stimulating the cells with a combination of phorbol-12-myristate 13-acetate (PMA) and anti-CD28 antibodies.
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