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Direct spectroscopic determination of functional sulphydryl groups on intact cell surfaces by surface-enhanced
B N Rospendowski1, J M Campbell, J Reglinski
1Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow, Scotland, UK.
European Biophysics Journal : EBJ
|January 1, 1992
Abstract:
Semi-quantitative and direct determination of labelled sulphydryl groups on the surface of intact erythrocytes has been accomplished for the first time with surface-enhanced resonance Raman scattering (SERRS). The method, which involves the use of citrate-reduced silver colloids, is sensitive and selective. A 10(-8) M effective concentration of picomole quantities of sulphydryl groups was determined in the presence of the normally overwhelming signal from haemoglobin. This seminal study suggests that SERRS may be applied to other in situ, site-directed labelling experiments.