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High resolution two-dimensional mapping of tissue-specific polypeptides in the desert locust, Schistocerca gregaria
R S Annadurai1, W Oberthür, H Rembold
1Max-Planck-Institut für Biochemie, Martinsried, Germany.
Electrophoresis
|July 1, 1992
Abstract:
High resolution two-dimensional gel electrophoresis (2-DE) using immobilized pH gradients was used to map the tissue-specific polypeptides of the desert locust, Schistocerca gregaria. Highly specific comprehensive 2-DE reference maps ("master gels") were developed for the brain, corpus cardiacum, subesophageal ganglion, and hemolymph. The polypeptides were well resolved within the pH 4-7 range in the first dimension and within the 14-94 kDa molecular mass range in the second dimension.