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Elimination of sample diffusion and lateral band spreading in isoelectric focusing employing ready-made immobilized
1Institut für Mikrobiologie, Technische Universität München, Germany.
Electrophoresis
|July 1, 1992
Abstract:
A simple procedure for the elimination of lateral sample as well as band spreading in precast, ready-made immobilized pH gradient gels is described. Round or rectangular holes are punched in the dry polyacrylamide gels prior to rehydration. The generated wells proved suitable for application of samples containing surfactants such as Nonidet P-40 or 3-[(3-cholamidopropyl) dimethylammonio]-1- propane-sulfonate (CHAPS). Lateral band spreading and precipitation of samples containing up to 9.5 M urea could be completely eliminated by this method.