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Related Experiment Videos

Denitrification by fungi.

H Shoun1, D H Kim, H Uchiyama

  • 1Institute of Applied Biochemistry, University of Tsukuba, Japan.

FEMS Microbiology Letters
|July 15, 1992
PubMed
Summary
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Many fungi possess the unexpected ability to perform denitrification, converting nitrite into various nitrogen gases like nitric oxide and nitrous oxide. This discovery reveals novel fungal capabilities in nitrogen cycling.

Area of Science:

  • Microbiology
  • Environmental Science
  • Mycology

Background:

  • Fungi, particularly Fusarium species, are increasingly recognized for their diverse metabolic capabilities.
  • Denitrification, the microbial process of reducing nitrate or nitrite, is crucial for nitrogen cycling in ecosystems.
  • The role of fungi in denitrification has been historically underestimated.

Purpose of the Study:

  • To investigate the denitrification potential of fungi within the Fusarium group and their teleomorphs.
  • To identify the specific nitrogenous products formed during anaerobic reduction of nitrite and nitrate by these fungi.
  • To characterize the mechanism of dinitrogen formation in fungal denitrification.

Main Methods:

  • Anaerobic incubation of fungal strains with nitrite or nitrate as substrates.

Related Experiment Videos

  • Gas chromatography and mass spectrometry to identify and quantify gaseous products (nitric oxide, nitrous oxide, dinitrogen).
  • Isotope labeling studies using [15N]nitrite to trace nitrogen pathways.

    • Main Results:

    • Many Fusarium and related fungal strains demonstrated the ability to reduce nitrite anaerobically, producing nitric oxide (NO), nitrous oxide (N2O), and/or dinitrogen (N2).
    • Nitrous oxide was the predominant product from nitrate or nitrite reduction, with some strains also producing N2.
    • Isotopic analysis confirmed N2 formation and revealed the production of a unique 'hybrid' dinitrogen (14N15N) from [15N]nitrite, indicating a novel denitrification pathway.

    Conclusions:

    • A significant number of fungi, especially within the Fusarium group, possess denitrifying abilities.
    • Fungal denitrification contributes to nitrogen cycling and can produce various nitrogen gases, with N2O being a major product.
    • Fungal denitrification involves unique biochemical reactions, including the formation of hybrid dinitrogen, warranting further investigation.