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Related Experiment Videos

Conjugative trimethoprim resistance in Staphylococcus aureus.

E E Udo1, M Q Wei, W B Grubb

  • 1School of Biomedical Sciences, Curtin University of Technology, Perth, W.A.

FEMS Microbiology Letters
|October 15, 1992
PubMed
Summary

A novel conjugative plasmid, pWBG707, from multiply resistant Staphylococcus aureus facilitates high-frequency transfer of trimethoprim and kanamycin resistance. This discovery advances understanding of antibiotic resistance mechanisms in bacteria.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Multiply resistant Staphylococcus aureus poses a significant public health threat.
  • Understanding the genetic basis of antibiotic resistance is crucial for developing effective treatments.

Purpose of the Study:

  • To characterize the plasmids harbored by a multiply resistant Staphylococcus aureus isolate (WBG7410).
  • To investigate the mechanism of trimethoprim and kanamycin resistance transfer.

Main Methods:

  • Plasmid isolation and characterization.
  • Conjugation experiments to assess resistance transfer.
  • Restriction endonuclease analysis (EcoRI) of plasmid DNA.

Main Results:

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  • Isolate WBG7410 contains plasmids of 38, 26, 2.8, 2.4, and 1.9 kb.
  • Trimethoprim and kanamycin resistance were transferred at high frequencies.
  • The 38-kb plasmid, pWBG707, is conjugative, encodes trimethoprim resistance, and mobilizes a 2.8-kb plasmid (likely encoding kanamycin resistance).
  • pWBG707 appears to be a novel conjugative plasmid in S. aureus.

    • Conclusions:

    • Plasmid pWBG707 is a key element in the dissemination of trimethoprim and kanamycin resistance in this S. aureus strain.
    • The identification of pWBG707 provides new insights into plasmid-mediated antibiotic resistance in Staphylococcus aureus.