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CORRELATION OF FLUORESCENT ANTIBODY, HISTOPATHOLOGY, AND CULTURE ON TISSUES FROM 372 ANIMALS EXAMINED FOR
Journal of Bacteriology
|March 1, 1965
Summary
Diagnosing histoplasmosis and blastomycosis in animals requires combining fluorescent antibody (FA) testing, histopathology, and culture methods. No single technique alone provides sufficient diagnostic accuracy for these fungal diseases.
Area of Science:
- Veterinary Mycology
- Diagnostic Microbiology
- Infectious Diseases
Background:
- Histoplasmosis and blastomycosis are significant fungal infections in animals.
- Accurate diagnosis is crucial for effective treatment and public health.
Purpose of the Study:
- To evaluate the diagnostic efficacy of fluorescent antibody (FA) technique, histopathology, and culture for detecting Histoplasma capsulatum and Blastomyces dermatitidis in animal tissues.
- To determine the optimal combination of diagnostic methods for mycosis detection.
Main Methods:
- Tissues from 372 animals across 16 species were examined using culture, FA technique, and histopathology.
- Fungal identification was confirmed by isolation or demonstration in tissue sections/exudates.
Main Results:
- Of 372 animals, 34 had blastomycosis and 21 had histoplasmosis; 300 were negative by all methods.
- Histopathology showed the highest sensitivity for blastomycosis (94%), while culture was highest for histoplasmosis (67%).
- FA technique sensitivity was 74% for blastomycosis and 71% for histoplasmosis.
Conclusions:
- Individual diagnostic methods (FA, histopathology, culture) are insufficient for accurate diagnosis of histoplasmosis and blastomycosis.
- Employing all three techniques concurrently yields the most satisfactory and reliable diagnostic results for these animal mycoses.